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What is XCR®

Xtreme Chain Reaction or XCR® is a patented ultra-fast, DNA/RNA amplification method and assay design technology using a unique combination of thermal cycling, primer design and template selection. This unique chemistry prevents artifact formation, e.g. “primer-dimer”, thereby eliminating false positive tests and improving sensitivity. By using standard enzymes and reagents, XCR® is immediately adaptable to existing PCR instruments, reagents and probe technologies.

It has many benefits including:

Ease‐of‐Use ‐XCR® chemistry allows for the development of simple/inexpensive instrumentation that does not require precise thermal control. Longer amplicon lengths provide for greater design space for XCR® assays. Coupled with XCR® ’s amplification speed, assay design and optimization time is reduced from 1 week to 1 day.
Affordability - By eliminating amplification artifacts, XCR® enables a return to off-patent, double stranded DNA binding dyes (i.e. SYBR). XCR® performs on existing platforms so that no new capital investment is required. XCR® -enabled novel instruments are lower cost; Fluoresentric has prototyped an instrument that uses battery power and off-the-shelf plastic consumables.
Performance - XCR® is 5- to 10-fold faster than PCR, forms no amplification artifacts, has single copy sensitivity and displays innate resistance to classic PCR inhibitors. XCR® has demonstrated high multiplex amplification capability.
There’s More Coming ‐ - More IP is in the works with this magnificent chemistry so stay tuned.

It is easy to adopt:

XCR® is readily adopted by PCR users with a promise to significantly improve the efficiency of existing and next generation systems. It uses off‐the‐shelf reagents and consumables while functioning on the entire installed base of PCR platforms. Users can adopt XCR® to work in concert with PCR to improve existing tests/methods, produce XCR® based tests de novo, or can replace PCR tests with XCR® to produce superior results. XCR® ’s simplicity and elegance lie in taking advantage of temperature ranges not utilized by PCR. Additionally, XCR® design and optimization methods are codified, enabling web based assay design, facilitating rapid adoption.

Applications include:

LDT/RUO ‐ XCR® can replace existing PCR‐based tests, or work in conjunction with PCR to make it better.
Field Based/Near Patient ‐ XCR® ’s reaction time of ~5 minutes makes possible the realization of PoC testing without compromising cost, sensitivity or accessibility.
Genetic Testing ‐ XCR® offers this market the most efficient process to conduct quantification/genotyping with a one sample one reaction solution.
Industrial Testing ‐ With a clear regulatory pathway, large and recurring testing volumes and time advantages compared to current methods, XCR® ’s codified design methods, shorter optimization runs and lower material costs bring this market into play.
Next Generation Sequencing (NGS) – XCR® eliminates enrichment bias by covering the entire range of GC content reaching areas within the genome PCR is unable to. XCR® performs more efficiently at up to 400 base pairs of DNA providing greater data density in assembly accelerating processing time.