Principle of XCR®
XCR® is more efficient than traditional PCR methods due to the way in which assay design and thermal amplification profile are approached. Rather than simply boiling a sample and expecting all of the nucleic acid in the sample to denature and fall apart, XCR® uses explicitly designed denature temperatures to minimize the possibility of amplification primers binding to non-target regions.
This approach makes the amplification more efficient from a time perspective, compared to PCR’s thermal cycling need to traverse the full traditional range of ~40C. Furthermore, the efficiency of the actual reaction itself is enhanced by reducing the formation of non-specific product and allowing the building blocks (dNTPs) and the enzymes to focus on the formation of the desired target amplicons.