Assay Design & Confirmation Services

Since 2003, Fluoresentric’s (now XCR Diagnostics’) custom assay design and confirmation services have delivered over 1,000 PCR assays for genetic testing entities including: government, biotechnology, biopharmaceutical, pharmaceutical, clinical diagnostic, veterinary diagnostic, and food manufacturing organizations.

Today, XCR Diagnostics leverages Fluoresentric's extensive experience, utilizing its award winning proprietary nucleic acid amplification chemistry, Xtreme Chain Reaction (XCR^®) to custom design your target of choice optimized on your existing PCR instruments.

XCR^® assays have been optimized on the following PCR instruments:

3M Integrated Cycler
Roche Light Cycler 2.0
Roche Light Cycler 480
Applied Biosystems StepOne
Bio-Rad CFX96
Cepheid SmartCycler l

Roche Light Cycler 96
Roche Light Cycler 1.2
Applied Biosystems 7500 Fast
Qiagen Roto-Gene Q 5plex HRM
DxNA Genestat
Cepheid SmartCycler ll

Confirmation Services include:

Sequences of primers designed to meet your assay needs
Sequence(s) of the fluorescent probe(s)
Oligonucleotide sequence(s) BLAST data to demonstrate design specificity
Oligonucleotide alignment on target template
Amplification protocol (thermal cycling and buffer conditions)
Optimized to your instrument of choice
Test kits provided for validation